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Water for AFM Imaging

Page history last edited by Dmitry Sokolov 11 years, 2 months ago

http://nanoscaleworld.bruker-axs.com/nanoscaleworld/forums/p/1322/3634.aspx

The water I use is "water for molecular biology", the item code for this is W4502.

If this differs from W3513, I cannot be sure, it looks quite similar on the sigma site. But I have tried another water they sold "water for cell culture" W3500, and it was not as good as W4502.

Sigma W4502 is recommended without blowing the water out. Just let the droplet dry in air.

To avoid possible contamination, tilt the substrate and shake the water off the substrate. The sample can be rinsed with the flash of water and the sample tilted on 45 degrees and facing a sink or a rubbish bin if needed. The sample can be dried for 5 minutes under a Petri dish to avoid contamination from air, or it can be heated to 60-80 Celsius if time is critical and sample is not temperature-sensitive.

Drying sample with a (nitrogen) air gun may lead to the deposition of particles. Dry gas can only remove excess of water from the surface. Complete removal of water thin film requires the sample heating well above the boiling point.