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Living Cells Imaging AFM

Page history last edited by Dmitry Sokolov 9 years, 12 months ago

AFM Careful Imaging


Cantilevers for Imaging Living Cells  


Living Cells Imaging with ICON AFM


Re: Multimode AFM and cell
By Ang Li in SPM Digest

 

Hi, Ashkan,

if your cells measured 3-4um high in air, they could be higher in liquid and that's pretty challenging for normal setup. Also tapping mode doesn't help to reduce lateral force in such case since the side wall of the tip or even cantilever will actually hit the cells and move them away. ScanAsyst fluid tips are not optimized for fluid tapping, and they are pretty sharp and practically blunt tips would produce better images of living cells. You may wanna refer to Brukerprobe website for your selection of proper tip for your application following the link here: http://www.brukerafmprobes.com/t-faq.aspx

Peakforce QNM is a powerful quantitative mechanical property mapping technique introduced by Bruker recently. There are quite a few discussions in this forum on this technique and some of them particularly focus on biological applications. You can start by reading some of the app notes listed here:

http://nanoscaleworld.bruker-axs.com/nanoscaleworld/media/p/1548.aspx

http://nanoscaleworld.bruker-axs.com/nanoscaleworld/media/p/418.aspx

also please find here a nice summary from Adam on the unique beauties of the technique:

http://www.linkedin.com/groups/Anyone-used-PeakForce-Tapping-mode-2815697.S.48366474?qid=b4780d5d-4273-44eb-a5af-15338ab48d67&trk=group_most_popular-0-b-ttl&goback=%2Egmp_2815697

LA

 

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