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Fluorescence Microscopy Applications

Page history last edited by Dmitry PNGHS 10 years, 3 months ago

Fluorescence Microscopy Applications

 

Please use this page to arrange data on FM and LM related chemicals.

Fluorescence Microscopy Principles is a good starting point for new in this technique.

 

Good source of information on staining: http://stainsfile.info/StainsFile/jindex.html

and

http://www.spectra.arizona.edu/ (supercedes http://www.mcb.arizona.edu/ipc/fret/ )

and

http://sylvester.org/research/shared-resources/laboratory-resources/analytical-imaging-core-facility/analytical-imaging-core-facility-links-forms/pubspectra-data   (supercedes http://home.earthlink.net/~pubspectra/ ) (download the zip file cited in the fn Excel data file with over 2,000 spectra).

and

http://www.photochemcad.com/pages/chemcad/chem-home.html (Program with 150 fluorophore spectra and calculator).

and

http://www.biocompare.com/Default.aspx

http://www.sigmaaldrich.com/analytical-chromatography/analytical-reagents/microscopy/stains-and-dyes.html 

 

Please refer to Spectra Viewers Web page for information on fluorescence spectra.

 

FM imaging procedure example.

 

Based initially on data provided by Olympus mirrorunits.pdf

Fluorescent protein intormation:

http://www.olympusfluoview.com/applications/fpcolorpalette.html

For more information on stains and for ordering visit:

Sigma or Invitrogen

More references on Leica fluorescence cubes can be found here:

http://www.leica-microsystems.com/lmsaccessories.nsf/showfluochromelist?openagent

http://www.fluorescence-microscopy.com/LMSAccessories.nsf/showFilterlist

 

Hazards information

Callose Staining

http://commonweb.unifr.ch/biol/pub/mauchgroup/staining.html

Aniline blue

Cellulose Staining

http://commonweb.unifr.ch/biol/pub/mauchgroup/staining.html

Calcofluor

Chitin Staining

http://commonweb.unifr.ch/biol/pub/mauchgroup/staining.html

Calcofluor

Dead cells staining

Ethidium Bromide

545nm 605nm     Cell membrane impermeability

Ethidium homodimer-1

528nm     617nm     Cell membrane impermeability

Propidium Iodide (PI)

530nm     615nm     Cell membrane impermeability

Trypan Blue

Cell membrane impermeability

http://tools.invitrogen.com/content/sfs/appendix/Cell_Culture/Viable%20Cell%20Counts%20Using%20Trypan%20Blue.pdf

Living Cells Staining

Acridine Orange

490nm     590nm     U-MWB2, U-MNB2, U-MSWB2          Acid organelle

BCECF/AM

500nm     530nm     U-MWIB2, U-MWIBA2, U-MNIBA2     Esterase substrates

Calcein/AM

495nm     520nm     U-MWIB2, U-MWIBA2, U-MNIBA2     Esterase substrates

Carboxyfluorescein-diacetate (CFDA)

495nm     520nm     U-MWIB2, U-MWIBA2, U-MNIBA2     Esterase substrates

http://flowbook.denovosoftware.com/Flow_Book/Chapter_9:_Cell_Death,_Including_Apoptosis

Rapid and reliable technique for specifically staining intact chloroplast

http://www.jhc.org/cgi/content/full/52/5/701

CYTO9

http://pp.physik.uni-osnabrueck.de/SeminarSS07/vortrag_Meschke180607.pdf

Fluoresceindiacetate (FDA)

495nm     520nm     U-MWIB2, U-MWIBA2, U-MNIBA2     Esterase substrates

http://flowbook.denovosoftware.com/Flow_Book/Chapter_9:_Cell_Death,_Including_Apoptosis

 

Pollen Tube Staining

http://commonweb.unifr.ch/biol/pub/mauchgroup/staining.html

Aniline blue 

Mitochondrial Dyes

http://www.bioxys.com/i_Biotium/mitochondrial_dyes.htm

http://www.med.unipg.it/imagelab/mitoch.html

 

Fluorochrome Excitation maximum (nm) Emission maximum (nm) Applicable mirror unit Comments Available Papers, links, references
Green Fluorescent Protein variants            
GFP (WT) 395, 475 510 U-MWIB2, U-MWIBA2, MNIBA2, U-MGFPHQ      
GFP (S65T) 488 510 U-MWIB2, U-MWIBA2, U-MNIBA2, U-MGFPHQ      
EGFP 488 507 U-MWIB2, U-MWIBA2, U-MNIBA2, U-MGFPHQ      
EBFP 380 440 U-MWU2, U-MNU2, U-MNUA2      
EYFP 513 527 U-MWIB2, U-MWIBA2,U-MNIBA2, U-MYFPHQ      
ECFP 433, 453 475, 501 U-MWBV2, U-MNBV2, U-MCFPHQ      
RFP (DsRed) 558 583 U-MWIG2, U-MWIY2      

Venus

515 528       http://www.olympusfluoview.com/applications/fpcolorpalette.html
Carbohydrate modification, protein modification (labeling)            
Fluorochrome antibody labeling            
Alexa Fluor 350 346 442 U-MWU2, U-MNU2, U-MNUA2      
Alexa Fluor 430 431 541 U-MWBV2, U-MNBV2      
Alexa Fluor 488 495 519 U-MWIB2, U-MWIBA2, U-MNIBA2      
Alexa Fluor 532 531 554 U-MWIB2, U-MWIBA2, U-MNIBA2      
Alexa Fluor 546 556 573 U-MWIG2      
Alexa Fluor 568 578 603 U-MWG2, U-MNG2, U-MWIG2      
Alexa Fluor 594 590 617 U-MWIY2      
Allophycocyanin (APC) 650 660 U-MWIY2      
7-amino-4-methylcoumarin-3-acetic acid (AMCA) 350 450 U-MWU2, U-MNU2, U-MNUA2      
BODIPY FL 503 512 U-MWIB2, U-MWIBA2, U-MNIBA2      
Cascade Blue 376 425 U-MWU2, U-MNU2, U-MNUA2      
Fluorescein-isothiocyanate (FITC) 490 520 U-MWIB2, U-MWIBA2, U-MNIBA2      
Oregon Green 488 496 520 U-MWIB2, U-MWIBA2, U-MNIBA2      
Phycoerythrin B (PE-B) 545 576 U-MWG2, U-MNG2, U-MWIG2      
Phycoerythrin R (PE-R) 490, 565 578 U-MWIG2, U-MNG2, U-MWIB2      
Rhodamine B-isothiocyanate (RITC) 570 595 U-MWG2, U-MNG2, U-MWIG2      
Rhodamin-Phalloidin         MMIC (-20C)  
Texas Red 596 620 U-MWIY2      
Tetramethylrhodamine-isothyocyanate (TRITC) 541 572 U-MWIG2      
LaserPro IR 790 785 800 *1      
Protein modification            
8-Anilino-1-naphthalene sulfonic acid Mg salt (ANS) 385 485 U-MWU2, U-MNU2, U-MNV2 Hydrophobic domain    
Coumarin maleimide (CPM) 385 465 U-MWU2, U-MNU2 Thiol group    
Cy2 489 506 U-MWIB2, U-MWIBA2, U-MNIBA2 Amino group, antibody labeling    
Cy3 552 565 U-MWIG2 Amino group, antibody labeling    
Cy 3.5 581 596 U-MWIY2 Amino group, antibody labeling    
Cy 5 650 667 U-MWIY2 Amino group, antibody labeling    
Cy 5.5 675 694 *1 Amino group, antibody labeling    
Cy 7 743 767 *1 Amino group, antibody labeling    
Dimethylaminonaphthalene-5-sulfonic acid (DANS)  380 520 U-MWU2, U-MNU2 Thiol group    
Dansyl chloraide 335 500 U-MWU2, U-MNU2 Amine labeling    
Dansyl hydrazine 336 531 U-MWU2, U-MNU2 Carbonyl group    
Eosin-isothiocyanate 524 548 U-MWIB2, U-MWIBA2, U-MNIBA2 Amine labeling    
Fluorescamine 390 460 U-MWU2, U-MNU2, U-MNV2 Amine labeling    
FluorX 494 520 U-MWIB2, U-MWIBA2, U-MNIBA2 Amino group, antibody labeling    
Lucifer Yellow         MMIC  
Lucifer Yellow CH 435 530 U-MWBV2, U-MNBV2, U-MWB2 Aldehyde, Ketone    
Monochlorobimane 380 461 U-MWU2, U-MNU2, U-MNUA2 Thiol group    
o-Phtalaldehyde (OPT) 340 455 U-MWU2, U-MNU2, U-MNUA2 Amine labeling    
Resorufin 570 585 U-MWG2, U-MNG2, U-MWIG2 Thiol group    
SITS 350 420 U-MWU2, U-MNU2, U-MNUA2 Amine labeling    
Carbohydrate modification            
Acriflavine SO2-PAS 430 515 U-MWBV2, U-MNBV2 Sugar    
Nuclei, nucleic acid staining            
Nuclei, nucleic acid            
Acridine Orange 490 530, 640 U-MWB2, U-MNB2 Single/double-stranded nucleic acid MMIC  
Acridine Yellow 470 550 U-MWB2, U-MNB2 Nucleic acid    
Acriflavine-Feulgen 430 515 U-MWBV2, U-MNBV2 DNA    
Auramine O-Feulgen 460 550 U-MWB2, U-MNB2 DNA    
7-Amino Actinomycin D 555 655 U-MWIY2, U-MWIG2 Probe for G-C rich regions    
bis-Aminophenyl-oxadizole (BAO) -Feulgen 380 470 U-MWU2, U-MNU2, U-MNUA2 DNA    
BOBO 1 462 481 U-MWBV2, U-MNBV2, U-MWB2 DNA    
BO PRO 1 462 481 U-MWBV2, U-MNBV2, U-MWB2      
Chromomycin A3 450 570 U-MWB2, U-MNB2, U-MWIB2 Probe for G-C rich regions    
4, 6-diamidino-2-phenyl-indole HCI (DAPI) 372 456 U-MWU2, U-MNU2, U-MNUA2 Probe for A-T rich regions  MMIC  
Ethidium Bromide 545 605 U-MWG2, U-MNG2, U-MWIG2 DNA/RNA    
Hoechst 33258, pentahydrate (bis-benzimide) 365 465 U-MWU2, U-MNU2, U-MNUA2 Probe for A-T rich regions; popular nuclear counterstain that emits blue fluorescence when bound to dsDNA    Its uses are similar to Hoechst 33342 for counterstaining, apoptosis and cell cycle studies, but Hoechst 33258 is reportedly less cell-permeant
Hoechst 33342 355 465 U-MWU2, U-MNU2, U-MNUA2 Probe for A-T rich regions    
Mithramycin 395 570 U-MWBV2, U-MNBV2 Probe for G-C rich regions    
Olivomycin 430 545 U-MWBV2, U-MNBV2 Probe for G-C rich regions    
Pararosaniline-Feulgen 560 625 U-MWG2, U-MNG2, U-MWIG2 DNA    
POPO 1 434 456 U-MWBV2, U-MNBV2      
Propidium Iodide (PI) 530 615 U-MWG2, U-MNG2, U-MWIG2 DNA/RNA    
Pyronin Y 540 570 U-MWIG2 DNA/RNA MMIC  
Quinacrine Mustard 385 525 U-MWBV2, U-MNBV2 Probe for A-T rich regions, Q band    

Saponin

        MMIC  

Serva Blue

        MMIC  

Sudan III

        MMIC  

Sudan IV

        MMIC  

Sudan Blue

        MMIC  
SYTOX Green nucleic acid stain 504 523 U-MWIB2, U-MWIBA2, U-MNIBA2      
Thiabendazole         MMIC  
Thiazole Orange 509 533 *1      

Thymidine

        MMIC  

Thymol Blue

        MMIC  
TOTO 1 514 533 *1 DNA    
TOTO 3 642 661 *1      
TO PRO 3 642 661 *1      

Trypan Blue

        MMIC  
YO PRO 1 491 509 U-MWIB2, U-MWIBA2, U-MNIBA2      
YOYO 1 490 510 U-MWIB2, U-MWIBA2, U-MNIBA2 DNA    
Membranes, organelle, membrane potential            
Membranes            
8-Anilino-naphthalene sulfonic acid (ANS) 385 485 U-MNV2 Marker for negatively loaded domain    
Dil 550 565 U-MWG2, U-MNG2, U-MWIG2 Marker for positively loaded domain, tracer    
DiO 484 501 U-MWB2, U-MNB2, U-MWIB2 Marker for positively loaded domain, tracer    
Diphenylhexatriene (DPH) 351 430 U-MWU2, U-MNU2, U-MNUA2 Neutral    
Filipin 350 U-MWU2, U-MNU2, U-MNUA2 Cholesterol    
Fluorescamine 390 460 U-MWU2, U-MNU2, U-MNV2 Membrane surface protein    
Merocyanine 540 500 572 U-MWG2, U-MNG2, U-MWIG2 Voltage sensitive dye    
Organelle            
Acridine Orange 490 590 U-MWB2, U-MNB2, U-MWIB2 Lysosome, nucleus    
BODIPY FL Ceramide 503 512 U-MWIB2, U-MWIBA2, U-MNIBA2 Golgi apparatus    
DiO C6(3) 480 501 U-MWIB2, U-MWIBA2, U-MNIBA2 Endoplasmic reticulum    
ERTracker Blue-White DPX 374 575 U-MWU2, U-MNU2, U-MNV2 Endoplasmic reticulum    
ER tracker Red         MMIC (-20C)  
JC-1 514 529 U-MWIB2, U-MWIBA2, U-MNIBA2 Mitochondria    
LysoTracker Red DND-99 577 590 U-MWIG2, U-MWIY2 Lysosome    
MitoFluor Green 489 517 U-MWIB2, U-MWIBA2, U-MNIBA2 Mitochondria    
Mitotracker Green FM         MMIC (-20C)  
MitoTracker Red CMXRos 578 599 U-MWIG2, U-MWIY2 Mitochondria  MMIC (-20C)  
Nile Red 485-530 525-605 U-MWB2, U-MNB2, U-MWIB2 Lysosome  MMIC  
Rhodamine 123 500 540 U-MWIB2, U-MWIBA2, U-MNIBA2 Mitochondria    
Pyronin Y 540 570 U-MWIG2 Mitochondria    
Membrane potential            
Di-4-ANEPPS 440/505 705 *1 Ratio metric    
Di-8-ANEPPS 436/546 713 *1 Ratio metric    
FM 1-43 488 520 *1 Synaptic vesicle    
FM 1-43FX         MMIC  
RH160 577 >610 *1      
RH414 520 >610 *1      
RH421 541 >590 *1      
RH423 520-550 >610 *1      
RH795 560 650 *1      
WW781 633 >645 *1      
Bacteria staining            
Bacteria            
Acridine Orange 490 530, 640 U-MWB2, U-MNB2, U-MWIB2 Myobacterium tuberculosis    
Acridine Yellow 470 550 U-MWB2, U-MNB2, U-MWIB2 Myobacterium tuberculosis, acid-fast bacteria    
Auramine O 460 550 U-MWB2, U-MNB2, U-MWIB2 Myobacterium tuberculosis, acid-fast bacteria    
Berberine sulfate 430 550 U-MWBV2, U-MNBV2 General bacteria    
Coriphosphine O 460 575 U-MWB2, U-MNB2, U-MWIB2 Bacterial cell wall    
Hoechst 33258 365 465 U-MWU2, U-MNU2, U-MNUA2 Mycoplasma detection    
Bone            
Ion concentrations            
Calcium            
bis-Fura-2 340/380 510 *1 2-wavelength excitation    
BTC 400/480 540 *1 2-wavelength excitation    
Calcium Green 505 532 *1 1-wavelength    
Fluo 3 485 503 *1 1-wavelength    
Fura-2 340/380 510 *1 2-wavelength excitation    
Indo-1 350 405/480 *1 2-wavelength emission    
Oregon Green 488 BAPTA-1 497 517 *1 1-wavelength    
Oregon Green 488 BAPTA-5N 497 517 *1 1-wavelength    
pH            
BCECF 430/480 530 *1 2-wavelength excitation    
Carboxy SNARF-1 530 580/630 *1 2-wavelength emission    
Nerve cell tracer, others            
Nerve cell tracer            
Evans Blue 550 610 U-MWG2, U-MNG2, U-MWIG2 Retrograde labeling MMIC  
Fast Blue 360 410 U-MWU2, U-MNU2, U-MNUA2 Retrograde labeling    

Fast Blue salt RR

        MMIC  
Granular Blue 375 410 U-MWU2, U-MNU2, U-MNUA2 Retrograde labeling    
True Blue 373 404 U-MWU2, U-MNU2, U-MNUA2 Retrograde labeling    
Others            
2-aminoacridone, hydrochloride        

glycosaminoglycan

 

 

Aceto-carmine

        MMIC  

Alcian Blue

        MMIC  
Aniline Blue, Aqueous     U-MWU2, U-MNU2, U-MNUA2 Cellulose, gydrogen-ion indicator MMIC  Gurr 1960
             
Alexa Fluor 488-Phalloidin 495 519 U-MWIB2, U-MWIBA2, U-MNIBA2 Actin fibers    

Azure II

        MMIC  

Brilliant Cresyl Blue ALD

        MMIC  
Calcofluor       Chitin  MMIC  
Calcofluor White 440 500-520 U-MWU2, U-MNU2, U-MNV2 Cellulose    Water soluble
cADP-ribose - Cyclic adenosine 5'-diphosphate ribose        gangliosides  

 

 

CDCFDA         MMIC (-20C)  

Coomassie Brilliant Blue

        MMIC  

Dimethyl Yellow

        MMIC  
DiOC6(3) - 3,3'-dihexyloxacarbocyanine iodide       mitochondria of live cells, glycogen  MMIC "Nuclear assembly with lambda DNA in fractionated Xenopus egg extracts: an unexpected role for glycogen in formation of a higher order chromatin intermediate." Hartl P, Olson E, Dang T, Forbes DJ. J Cell Biol 124, 235-248 (1994) PN17445.
Eosin       Plan tissues   Stable, Gurr 1960

Eosin Yellowish

        MMIC  
Euchrysine (Acridine Orange)     U-MWU2, U-MNU2, U-MNUA2 Cellulose    
Fast Green       Protein MMIC  
Fluorescein 494 521     MMIC http://en.wikipedia.org/wiki/Fluorescein, Gurr 1960
Fluorescein + potassium dichromate 494     Plan tissues    

5-Fluorodeoxyuridine

        MMIC  

Formic acid

        MMIC  

Fuchsin basic

        MMIC  

Gentian Violet

        MMIC  

Germanium Dioxide

        MMIC  

Giemsa Stain

        MMIC  

Glycerol

        MMIC  

Immersion Oil

        MMIC  

Iodine

        MMIC  

Janus Green

        MMIC  

Lactoglycerine

        MMIC  

Lithium Heparin

        MMIC  
Lucifer Yellow CH 430 535 U-MWBV2, U-MNBV2 Tracer    

Methyl Red

        MMIC  

Methylene Blue

        MMIC  

Neutral Red

        MMIC  
NanoOrange 485 590 U-MWIG2, U-MWG2, U-MWIB2 Protein quantification    
NBD-Phallacidin 465 530 U-MWIB2, U-MWIBA2, U-MNIBA2 Actin fibers    

Nigrosin

        MMIC  
Nile Blue 488 566   Fats MMIC  
Orcein       Elastin MMIC Gurr 1960, Gray 1954
Orcein       Basic dye MMIC Gurr 1960, Fullmer 1956
Orcein       Cytology MMIC Gurr 1960, 1% orcein in 45% propionic acid at squash technique for Carnoy-fixed material [Cotten 1959]

Phloroglucinol

        MMIC  
Primuline O 410 550 U-MWIG2, U-MWG2, U-MWIB2 Cellulose    
Rhodamine-Phalloidin 550 580 U-MWG2, U-MNG2, U-MSWG2 Actin fibers    

Ruthenium Red

      Pectin MMIC Sabba & Lulai, 2002
Sheridan       Elastin   Gurr 1960
Spectrum Aqua 433 480 U-MWBV2, U-MNBV2 FISH probe labeling    
Spectrum Gold     *1 FISH probe labeling    
Spectrum Green 497 524 U-MWIB2, U-MWIBA2, U-MNIBA2 FISH probe labeling    
Spectrum Orange 559 588 U-MWG2, U-MNG2, U-MWIG2 FISH probe labeling    
sulforhodamine 101 586 605 U-MWIY2 Tracer    
Thioflavin T 430 550 U-MWBV2, U-MNBV2 Amyloid    

Uridine

        MMIC  
Weigert       Elastin   Gurr 1960
WGA 488  570    Polysaccharides    
 Aniline Blue (Aqueous) Combinations            

Aniline Blue (Aqueous) in recipes:

Bensley's simple and triple contrast stains, Lendrum and McFarlane's quintuple contrast, Lillie's quadruple contrast, Milligan's triple stain, Paquin and Goddard's sextuple stain

 

      Algae, astrocytes, bacterial smears, centrosomes, filamentous fungi, fungus in tissue scrapings, gastric cells, kidney, pituitary, vaginal smears, plasmodesma, radulae, etc.   Gurr 1960, Gray 1954
Aniline Blue in Van Gieson, Masson-Van Giesson and Mason-Mallory-Van Gieson procedures       Collagen fibers, best results   Gurr 1960, Lillie 1945, best results compared to violamine R, maphthol blue black, fast green FCF, acid fuchsin, wool green S and methyl green
             
Aniline Blue - Niagara Blue 4B       Animal tissues   Better quality if Aniline Blue fails; Gurr 1960, Emig 1941
Aniline Blue - Safranin       Plan tissues: cellulose walls and achromatic figure   Gurr 1960, Johansen 1940
Aniline Blue - Eosin B (Rhodocyanin technique)       Anterior pituitary   Gurr 1960, Glenner 1957
Aniline Blue - Erythrosin or phloxine       filamentous Chlorophyta   Gurr 1960, Johansen 1940
Aniline Blue - Orange G - Gomori's chrome haematoxylin       Cell differentiation in hypoiphysis and pancreatic islets   Gurr 1960, Rona 1956
Congo Red - Aniline Blue - Orange G       Elastic fibres, fibrin, etc.   Gurr 1960, Gurr 1956
Haematoxylin - Ponceau S - Aniline Blue       Connective tissue and muscle   Gurr 1960, Gurr 1956
Masson's trichrome stain: haematoxylin - Ponceau Fuchsin - Aniline Blue       Connective tissues   Gurr 1960, Gurr 1956
Silver Carbonate - Orcein - Aniline Bolue - Fast Green FCF       Reticulin, elastin and collagen in the same tissue sections   Gurr 1960, Gurr 1956
Gomori's trichrome stain: haematoxylin - Fast Green - Neoponceau       Animal tissues   Gurr 1960, Gurr 1956
With Protargol, gallocyanin, fast gree FCF, orage G, etc.       Nerve fibres, sheaths and cells (Foley)   Gurr 1960, Gurr 1956
Improved Pasini stain with orcein, eosin and acid fuchsin       Connective tissue   Gurr 1960, Gurr 1956
Picroanilene blue       Fungal Mycelium in wood sections   Gurr 1960, Cartwright 1929
Picroanilene blue - Fast Green FCF - Orcein - Silver Ammonium Carbonate       Combined connective tissue for elastin, reticulin and collagen   Gurr 1960, Lewis 1951
Silver Ammonium Carbonate       Reticulin, orcein for elastin   Gurr 1960, Lewis 1951
Picroaniline Blue or Gast Green FCF       Collagen and other tissues   Gurr 1960, Lewis 1951

 

Antibodies for IF

Description Brand Ref # Comments Available Papers, links, references
* @-GFP-AF488 (1ary-conjugated, rabbit) Molecular Probe A21311   MMIC  
* TRITC-conjugated (goat anti-mouse) Sigma T5393   MMIC  
* AF488 (donkey anti-mouse) Molecular Probe A21202   MMIC  
* AF488 (donkey anti-rabbit) Molecular Probe A21206   MMIC (-20C)  
* AF555 (donkey anti-mouse) Molecular Probe A31570   MMIC (-20C)  
* AF555 (goat anti-rabbit) Molecular Probe A21428   MMIC  
* AF 568 (donkey anti-sheep) Molecular Probe A21099   MMIC (-20C)  
AF 568-Phalloidin Molecular Probe A12380   MMIC (-20C)  

 

Antifading agents and Mounting Media

Description Brand Ref # Comments Available

Papers, links, references

DPX Mountant

      MMIC  

 

 

Absorption and Emission Maxima for Common Fluorophores

FluorophoreAbsorption maximum (nm)Emission maximum (nm)
Acid fuchsin 540 630
ACMA 430 474
Acridine red 455-600 560-680
Acridine orange 500 (with DNA) 526 (with DNA)
  460 (with RNA) 650 (with RNA)
Acridine yellow 470 550
Acriflavin 436 520
AFA (acriflavin Feulgen SITSA) 355-425 460
Alizarin complexon 530-560 580
Allophycocyanin 650 660
AMCA-S, AMC 345 445
Aminoactinomycin D 555 655
7-Aminoactinomycin D (7-AAD) 546 647
Aminocoumarin 350 445
Anthroyl stearate 361-381 446
Astrazon brilliant red 4C 500 585
Astrazon orange R 470 540
Astrazon red 6B 520 595
Astrazon yellow 7 CLL 450 480
Atabrine 436 490
Auramine 460 550
Aurophosphine 450-490 515
Aurophosphine G 450 580
BAO 9 (bisaminophenyloxadiazole) 365 395
BCECF 482 (low pH) 520 (low pH)
  503 (high pH) 528 (high pH)
Berberine sulfate 430 550
Bisbenzamide 360 600-610
Blancophor FFG solution 390 470
Blancophor SV 370 435
BOBO 1, BO-PRO 1 462 481
BODIPY FL 505 513
BODIPY TMR 542 574
BODIPY TR 589 617
Brilliant sulfoflavin FF 430 520
Calcein 494 517
Calcein blue 370 435
Calcium crimson 590 615
Calcium green 506 533
Calcium orange 549 576
Calcofluor RW solution 370 440
Calcofluor white 440 500-520
Calcophor white ABT solution 380 475
Calcophor white standard solution 365 435
5-(and 6-)Carboxy SNARF-1 indicator 548 (low pH) 587 (low pH)
  576 (high pH) 635 (high pH)
6-Carboxyrhodamine 6G 525 555
Cascade blue 400 420
Catecholamine 410 470
CL-NERF 504 (low pH) 587 (low pH)
  514 (high pH) 540 (high pH)
Coriphosphine O 460 575
Coumarin-phalloidin 387 470
Cy3.18 554 568
Cy5.18 649 666
Cy7 710 805
DANS (1-dimethylamino-naphaline-5-sulfonic acid) 340 525
Dansyl NH-CH3 in water 340 578
DAPI 358 461
DiA 456 590
Diamino phenyl oxydiazole (DAO) 280 460
Di-8-ANEPPS 488 605
DiD [DiIC18(5)] 644 665
Dimethylamino-5-sulfonic acid 310-370 520
DiI [DiIC18(3)] 549 565
DiO [DiOC18(3)] 484 501
Diphenyl brilliant flavine 7GFF 430 520
DM-NERF 497 (low pH) 527 (low pH)
  510 (high pH) 536 (high pH)
Dopamine 340 490-520
ELF-97 alcohol 345 530
Eosin 524 544
Erythrosin ITC 530 558
Ethidium bromide 518 605
Euchrysin 430 540
FIF (formaldehyde-induced fluorescence) 405 433
Flazo orange 375-530 612
Fluorescein 494 518
Fluorescein isothiocyanate (FITC) 490 523
Fluo-3 506 526
FM1-43 479 598
Fura-2 363 (low [Ca2+]) 512 (low [Ca2+])
  335 (high [Ca2+]) 505 (high [Ca2+])
Fura Red 472 (low [Ca2+]) 657 (low [Ca2+])
  436 (high [Ca2+]) 637 (high [Ca2+])
Genacryl brilliant red B 520 590
Genacryl brilliant yellow 10GF 430 485
Genacryl pink 3G 470 583
Genacryl yellow 5GF 430 475
Gloxalic acid 405 460
Granular blue 355 425
Hematoporphyrin 530-560 580
Hoechst 33258, Hoechst 33342 352 461
3-Hydroxypyrene 5,8,10-trisulfonic acid 403 513
7-Hydroxy-4-methylcoumarin 360 455
5-Hydroxytryptamine (5-HT) 400 530
Intrawhite Cf liquid 360 430
Indo-1 346 (low [Ca2+]) 475 (low [Ca2+])
  330 (high [Ca2+]) 401 (high [Ca2+])
Leucophor PAF 370 430
Leucophor SF 380 465
Leucophor WS 395 465
Lissamine rhodamine B 570 590
Lucifer yellow CH 428 536
Lucifer yellow VS 430 535
LysoSensor blue DND-192, DND-167 374 425
LysoSensor green DND-153, DND-189 442 505
LysoSensor yellow/blue 384 (low pH) 540 (low pH)
  329 (high pH) 440 (high pH)
LysoTracker green 504 511
LysoTracker yellow 534 551
LysoTracker red 577 592
Magdala red 524 600
Magnesium green 506 531
Magnesium orange 550 575
Maxilon brilliant flavin 10 GFF 450 495
Maxilon brilliant flavin 8 GFF 460 495
MitoTracker green FM 490 516
MitoTracker orange CMTMRos 551 576
Mithramycin 450 570
MPS (methyl green pyronine stilbene) 364 395
NBD 465 535
NBD amine 450 530
Nitrobenzoxadidole 460-470 510-650
Noradrenaline 340 490-520
Nuclear fast red 289-530 580
Nuclear yellow 365 495
Nylosan brilliant flavin E8G 460 510
Oregon green 488 fluorophore 496 524
Oregon green 500 fluorophore 503 522
Oregon green 514 fluorophore 511 530
Pararosaniline (Feulgen) 570 625
Phorwite AR solution 360 430
Phorwite BKL 370 430
Phorwite Rev 380 430
Phorwite RPA 375 430
Phosphine 3R 465 565
Phycoerythrin R 480-565 578
Pontochrome blue black 535-553 605
POPO 1, PO-PRO 1 434 456
Primuline 410 550
Procion yellow 470 600
Propidium iodide 536 617
Pyronine 410 540
Pyronine B 540-590 560-650
Pyrozal brilliant flavin 7GF 365 495
Quinacrine mustard 423 503
R-phycoerythrin 565 575
Resorufin 570 585
RH 414 500 635
Rhodamine 110 496 520
Rhodamine 123 507 529
Rhodamine 5 GLD 470 565
Rhodamine 6G 526 555
Rhodamine B 540 625
Rhodamine B 200 523-557 595
Rhodamine B Extra 550 605
Rhodamine BB 540 580
Rhodamine BG 540 572
Rhodamine green fluorophore 502 527
Rhodamine red 570 590
Rhodamine WT 530 555
Rhodol green fluorophore 499 525
Rose Bengal 540 550-600
Serotonin 365 520-540
Sevron brilliant red 2B 520 595
Sevron brilliant red 4G 500 583
Sevron brilliant red B 530 590
Sevron orange 440 530
Sevron yellow L 430 490
SITS (primuline) 395-425 450
SITS (stilbene isothiosulfonic acid) 365 460
SNARF-1 563 639
Sodium green 507 535
Stilbene 335 440
Sulforhodamine B and C 520 595
Sulphorhodamine G Extra 470 570
SYTOX green nucleic acid stain 504 523
SYTO green fluorescent nucleic acid stains 494 ± 6 515 ± 17
SYTO green fluorescent nucleic acid stains 515 ± 7 543 ± 13
SYTO 17 red fluorescent nucleic acid stain 621 634
Tetracycline 390 560
Tetraethylrhodamine (rhodamine B) 555 580
Texas red 595 615
Thiazine red R 510 580
Thioflavin S 430 550
Thioflavin TCN 350 460
Thioflavin 5 430 550
Thiolyte 370-385 477-484
Thiozol orange 453 480
Tinopol CBS 390 430
TOTO 1, TO-PRO 1 514 533
TOTO 3, TO-PRO 3 642 660
True Blue 365 420-430
Ultralite 656 678
Uranine B 420 520
Uvitex SFC 365 435
X-Rhodamine 580 605
Xylene orange 546 580
XRITC 582 601
YOYO 1, YOYO-PRO 1 491 509
YOYO 3, YOYO-PRO 3 612 613

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Solvents

Name Available
Methanol MMIC
   

 

 

Other Chemicals

Name Brand Ref # Comments Available
Jasplakinolide Molecular Probes J7473   MMIC (-20C)
Latrunculin-A Molecular Probes L12370   MMIC (-20C)
Latrunculin-A 1mg/ml in DMSO Molecular Probes L12370   MMIC (-20C)

 

 

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